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  • ZFP57 and the Targeted Maintenance of …

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  • Demon King | Kono Subarashii Sekai ni Shukufuku wo! …

    2021-7-27 · The Demon King (real name Yasaka Kyouichi) is the main antagonist of the KonoSuba series, and is currently engaged in a war with Belzerg, and by extension the world at large. He resides in his castle, which has a powerful barrier protecting it, even being able to withstand the power of the Crimson Demon Clan. He is rumored to be incredibly powerful and ruthless, however, it seems that …

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    2014-11-17 · The amplified PCR products were purified by Wizard PCR SV Gel and PCR Clean-Up System kit (Promega, Madison, WI, USA) according to the manufacturer''s instruction and were sequenced using the BigDye ® Terminator v3.1 cycle sequencing kit (Applied Biosystems, Foster City, CA, USA) according to the manufactures instruction and those sequences ...

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  • Demon King Generals | Kono Subarashii Sekai ni …

    2021-7-26 · The Demon King''s generals are eight of the most powerful monsters or creatures in his army that maintain the barrier of the Demon King''s Castle through some unknown method and command his troops in the war against Belzerg. While all are affiliated with the Demon King and his army, some such as Wiz are merely Generals in title; while they still are quite powerful and maintain the barrier, they ...

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    After birth, the nongrowing (ng) oocytes start growing with follicle cells and become fully grown germinal vesicle (GV) oocytes. KDM1B removes H3K4 methylation at some maternally imprinted loci and Dnmt3a, 3b, 3l, and long noncoding RNAs (lncRNAs) are involved in …

  • A workflow for accurate metabarcoding using nanopore ...

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  • A workflow for accurate metabarcoding using …

    2021-1-28 · 3.3 Metabarcoding using Nanopore sequencing. Nanopore sequencing with the MinION delivered 746,153/2,756 and 499,453/1,874 1D/1D 2 reads for Protocols A and B (SRA PRJNA627498), respectively. The 1D approach only sequences one …

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    Kono is a Māori-owned, food and beverage company farming more than 1000ha of land and sea, and exporting to over 25 countries. Their brands include Tohu, Kono and Aronui wines, Tutū cider, Kono mussels, Kiwa oysters, Annies fruit bars and Hop Federation craft beer.

  • Maternal DNA Methylation Regulates Early …

    2016-1-25 · Critical roles for DNA methylation in embryonic development are well established, but less is known about its roles during trophoblast development, the extraembryonic lineage that gives rise to the placenta. We dissected the role of DNA methylation in trophoblast development by performing mRNA and DNA methylation profiling of Dnmt3a/3b mutants.

  • Molecular barcoding of native RNAs using nanopore ...

    2020-9-9 · Schematic overview of the direct RNA barcoding and demultiplexing strategy. (A) Overview of Oxford Nanopore library preparation protocol for native RNA sequencing.(B) Adaptation of A to include custom DNA barcodes.(C) Barcode segmentation and transformation, where the electric current associated with a barcode adapter (highlighted in red) is extracted and converted into an image using …

  • Inferring the choreography of parental genomes …

    Figure 1. Systematic representation of transcriptome analysis and summary of mapped read distributions. (A) Developmental stages targeted by the RNA-seq assay.(B) Distribution of uniquely mapped reads along mouse genomic features.(C,D) Histograms showing the number of RefSeq exons and introns and the coverage levels.(E) Histogram showing the number of intronic ncRNAs (coverage >0.6) and their ...

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